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JAEA Reports

Neutron biology for next generation; Report from J-PARC workshop "Neutron Biology for Next Generation"; March 22nd-23rd, 2017, Ibaraki Quantum Beam Research Center

Sugiyama, Masaaki*; Nakagawa, Hiroshi; Inoue, Rintaro*; Kawakita, Yukinobu

JAEA-Review 2017-024, 40 Pages, 2017/12

JAEA-Review-2017-024.pdf:8.69MB

Now-a-days, promotion of life science by utilizing neutron (neutrons biology) is highly demanded in our country, following installation and improvement of high quality and intensity neutron sources at J-PARC and JRR-3. Aiming at accelerating development of neutrons biology in our country, an international workshop "Neutron biology for next generation" was held as a J-PARC Workshop at Ibaraki Quantum Beam Research Center from 22 March to 23 March in 2017. In the workshop, latest instruments, new-fashioned methodologies, recent scientific results and future perspectives were extensively discussed by domestic neutron instrumental scientists and domestic/foreign neutron biologists. This is a report of the workshop summarized by organizers.

Journal Articles

On the dynamical approach of quantitative radiation biology

Ouchi, Noriyuki

Evolution of Ionizing Radiation Research, p.41 - 62, 2015/09

https://doi.org/10.5772/60859

Quantitative approach in radiation biology based on the clonogenic method and obtained cell survival curves as a dose-response relationship, are introduced. Generally, cell survival curves seem to have a universality on its function, i.e. functional form of survival curve seems to be unchanged under various conditions including different species. Various factors affecting the radiosensitivity have been introduced to find macroscopic nature of living organisms. Many mathematical models describing cell survival curves have been presented, however, functional form of cell survival curves derived from, based on biological mechanism does not yet exist. Finally, the possibility that the structural change of chromosome affects the repair process is discussed.

Journal Articles

Single-cell tracking and dynamical analysis of cellular population based on systems biology

Yokoya, Akinari; Kaminaga, Kiichi

Hoshasen Seibutsu Kenkyu, 49(4), p.418 - 431, 2014/12

no abstracts in English

Journal Articles

Study on evaluation of low-dose radiation effect based on molecular biology; Summarizing the comitted reserch

Saito, Kimiaki

Genankyo Dayori, (205), 11 Pages, 2005/04

http://www.nsra.or.jp/library/dayori/dayori_205.pdf

no abstracts in English

Journal Articles

Hydrogen and hydration in proteins

Niimura, Nobuo; Chatake, Toshiyuki; Kurihara, Kazuo; Maeda, Mitsuru

Cell Biochemistry and Biophysics, 40(3), p.351 - 369, 2004/06

https://doi.org/10.1385/CBB:40:3:351

Times Cited Count：24 Percentile：25.25(Biochemistry & Molecular Biology)

Neutron diffraction provides an experimental method of directly locating hydrogen atoms in proteins. High resolution neutron diffractometers dedicated to biological macromolecules (BIX-type diffractometer) have been constructed at the Japan Atomic Energy Research Institute (JAERI) and they have been used in the 1.5 AA -resolution crystal structure analyses of several proteins.

Journal Articles

Evaluation of the resistance of to ion beam radiation

Hayashi, Hirotaka*; Wada, Seiichi; Funayama, Tomoo; Narumi, Issei; Kobayashi, Yasuhiko; Watanabe, Hiroshi*; Furuta, Masakazu*; Uehara, Kaku*

Journal of Eukaryotic Microbiology, 51(3), p.321 - 324, 2004/06

https://doi.org/10.1111/j.1550-7408.2004.tb00573.x

Times Cited Count：5 Percentile：6.61(Microbiology)

no abstracts in English

Journal Articles

LET dependence of lethality of carbon ion irradiation to single tobacco cells

Yokota, Yuichiro; Hase, Yoshihiro; Shikazono, Naoya; Tanaka, Atsushi; Inoue, Masayoshi*

International Journal of Radiation Biology, 79(8), p.681 - 685, 2003/08

https://doi.org/10.1080/09553000310001622832

Times Cited Count：21 Percentile：78.43(Biology)

To determine the radiation sensitivity and the relationship between linear energy transfer (LET) and relative biological effectiveness (RBE) in single plant cells irradiated with heavy ions, single tobacco (BY-2) cells were irradiated with carbon ions (78.6 to 309 kev/m) and gamma rays (0.2 kev/m). Two weeks after the irradiation, colonies derived from the irradiated cells that had 16 cells or more were counted as survivors. The surviving fraction was fitted using the single-hit, multitarget theory. The dose needed to reduce the surviving fraction of the cells to 0.1 (D $_{10}$ ) of gamma rays and carbon ions were 47.2 and 10.5 to 12.6 Gy, respectively. The radiation sensitivity of single tobacco cells was much lower than that of mammalian cells, although the mean number of base pairs per chromosome in the two cell types is similar. The RBE based on the D $_{10}$ peaked at a LET of 247 keV/m. The RBE peak based on the D $_{10}$ of carbon ions in single tobacco cells occurred at a higher LET than it dose in other organisms.

Journal Articles

High resolution neutron protein crystallography, hydrogen and hydration in proteins

Niimura, Nobuo; Chatake, Toshiyuki; Ostermann, A.; Kurihara, Kazuo; Tanaka, Ichiro

Zeitschrift fr Kristallographie, 218(2), p.96 - 107, 2003/03

no abstracts in English

Journal Articles

Protein function prediction based on genome sequence

Yura, Kei

Puroteomikusu No Saishin Gijutsu, p.93 - 101, 2002/11

Determination of genome sequences has massive impacts on biology. Genome sequence is a blueprint of the species, and analyses of genome sequence may uncover every components of the species. However, when a whole genome of Haemophilus influenzae was sequenced, biologists realized that one was not able to deduce biological information out of the sequence. The genome sequence is full of information that knowledge based on conventional genetics and molecular biology can retrieve. Bioinformatics is the field that find niche in this area. In this chapter, we will overview the current status and the future of bioinformatics.

JAEA Reports

Radiation induced transformation; Article review

Saigusa, Shin*

JAERI-Review 2002-009, 43 Pages, 2002/03

JAERI-Review-2002-009.pdf:2.3MB

The mechanism of the process of radiation-induced transformation that leads normal mammalian cell to malignant feature is still not clear. However, a drastic development of molecular biological technique in medical biology research field since 1990's have progressively revealed the mechanisms of this process and recent results have been starting to incorporate in the recent radiation risk estimate procedures. This report describes the results of the survey and review of the articles concerned with experimental studies of radiation induced in vitro transformation, since 1993 to 1997.

Journal Articles

Neutron crystallography of hen egg-white lysozyme at pH4.9

Maeda, Mitsuru; Fujiwara, Satoru; Yonezawa, Yasushige*; Niimura, Nobuo

Journal of the Physical Society of Japan, Vol.70, Supplement A, p.403 - 405, 2001/05

no abstracts in English

JAEA Reports

Organic acid dissolving in groundwater in the Mobara gas field

JNC TN8400 2000-030, 17 Pages, 2000/12

JNC-TN8400-2000-030.pdf:0.44MB

As a representative of natural marine groundwater, the author selected pumped water from a Quaternary sedimentary aquifer of the Mobara gas-field in Japan and measured the concentration of total organic carbon (TOC) and of organic acid anions (formic, acetic, lactic, succinic, humic, fulvic, propionic, valeric and butyric acids). The concentration of TOC ranged from 221 to 240mg/L. As organic acid anions, only succinic and fulvic acids were detected and each concentration was given to be from 5.8 0.5 to 8.30.3 and from 3.30.2 to 3.50.2mg/L, respectively. By consideration of the temperature and the [SO $_{4}$ $^{2-}$ ] of the groundwater, it is inferred that the organic acid has been significantly decomposed by activities of microbes, such as the fermentation process, CH $_{3}$ COO $^{-}$ + H $_{2}$ O = HCO $_{3}$ $^{-}$ + CH $_{4}$ .